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About the Book

My goal for this document is to help you understand the theory behind 16S data processing. I want you to be able to do the analysis that makes the most sense for you. There are pipelines that make data analysis easy and simple. I aim to show you how the sausage is made. The entire data collection process, from sample collection to OTU calling, is discussed, with particular emphasis on preparing raw sequencing data for OTU calling and on selection of OTU calling methodology.


About the Author

Scott W. Olesen’s avatar Scott W. Olesen

Scott earned his PhD at MIT in 2016. His graduate work focused on creating conceptual and quantitative tools for analyzing microbiome data. He is now a Postdoctoral Fellow at the Harvard School of Public Health.

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