Quantification Manual for Volocity 6.3
Quantification Manual for Volocity 6.3
Getting your data more objective and faster!
About the Book
Volocity is an imaging analysis software developed by Perkin Elmer Company. It can be used for visualization, modification and quantitation of 2D images, 3D volumes or 4D volume sequences, including multi-channel images.
Volocity provides powerful tools for image quantification, which dramatically speed up data analysis. Besides, while manual counting prone to be subjective, automatic quantification by Volocity produces objective data with efficiently certain protocol. Volocity also provides modules for various functions, enabling fast establishment of novel protocol for specific aims. Also Volocity can process images such as remove noise and backgrounds, which can be employed to explore current imaging data to the utmost.
This manual contains basic skills about Volocity and emphasizes on quantification strategies. Detailed examples came from projects the author of this manual has worked with. Although this manual cannot cover every aspect of Volocity, it provides useful information on certain topics including finding and quantifying intensity of objects in 2D images as well 3D volumes, counting certain items and their colocalization and several image processing skills. Utilizing strategies in this manual, Volocity can analyze large amount of data within minutes, which means one can get results almost immediately after imaging. Strategies in this manual mainly came from the author’s trials, reflection, and exploration of Volocity User Guide for Volocity 6.3. They also showed the entire development process of current assays in use.
About the Author
Table of Contents
Finding Objects with Thresholding. 12
Finding Objects in Region of Interest (ROI). 15
Disturbance Removal with Filter Population in 2D images. 18
Disturbance Removal with Several Filters--Filter Population in 3D Images. 20
Operation between Population Sets. 23
Image Processing Functions and Measurements. 25
The Leanpub 60 Day 100% Happiness Guarantee
Within 60 days of purchase you can get a 100% refund on any Leanpub purchase, in two clicks.
Now, this is technically risky for us, since you'll have the book or course files either way. But we're so confident in our products and services, and in our authors and readers, that we're happy to offer a full money back guarantee for everything we sell.
You can only find out how good something is by trying it, and because of our 100% money back guarantee there's literally no risk to do so!
So, there's no reason not to click the Add to Cart button, is there?
See full terms...
Earn $8 on a $10 Purchase, and $16 on a $20 Purchase
We pay 80% royalties on purchases of $7.99 or more, and 80% royalties minus a 50 cent flat fee on purchases between $0.99 and $7.98. You earn $8 on a $10 sale, and $16 on a $20 sale. So, if we sell 5000 non-refunded copies of your book for $20, you'll earn $80,000.
(Yes, some authors have already earned much more than that on Leanpub.)
In fact, authors have earnedover $14 millionwriting, publishing and selling on Leanpub.
Learn more about writing on Leanpub
Free Updates. DRM Free.
If you buy a Leanpub book, you get free updates for as long as the author updates the book! Many authors use Leanpub to publish their books in-progress, while they are writing them. All readers get free updates, regardless of when they bought the book or how much they paid (including free).
Most Leanpub books are available in PDF (for computers) and EPUB (for phones, tablets and Kindle). The formats that a book includes are shown at the top right corner of this page.
Finally, Leanpub books don't have any DRM copy-protection nonsense, so you can easily read them on any supported device.
Learn more about Leanpub's ebook formats and where to read them
